Rating and seasonal sensitivity average effective dose in the comic spinulosus, using international standard of chorionic gonadotropin

Abstract

Using for the first time in our country international standard chorionic gonadotropin, we have observed seasonal changes in sensitivity to the release of sperm in the common toad Lima, in similar fashion to that presented various species of frogs in other countries. The results have appreciated the theoretical effective dose in IU for each station, values of 50 or finding DEM between 9 and 15 IU, with maximum sensitivity in spring, decreasing in summer and minimum in autumn, only to increase again in winter. Using these reports, as is understood, it is provided in our quantitative determination of human chorionic gonadotropin in international units using the Bufo spinulosus and using the dose-response curves for each station represented in a diagram, regardless of the time of year in which they work. However, we wish to state that the method is valid only for qualifications in blood and urine carried out in Lima; if you intend to apply it in other regions of the country, previously should be evaluated in each area the specific sensitivity of the reagent animal place to avoid possible miscalculation. Moreover, our laboratory animal has been more sensitive than their counterparts in other countries and comparable to the one with the rabbit, though less than the prepubescent rats. Therefore, we deem convenient to use the Bufo spinulosus for quantitative determinations when high levels of chorionic gonadotropin is seeking, while not suitable for low levels.