Embarazo mediante tratamiento de maduración in vitro de ovocitos en paciente amenorreica y con historia de anorexia nervosa

Luis Vargas Tominaga; Ricardo Pella Cáceres; Alberto Vargas Lechuga; Livia Bartolo Durán

doi:10.31403/rpgo.v58i86

Authors

Luis Vargas Tominaga Centro de Fertilidad y Ginecología del Sur. Médico Ginecólogo-Obstetra.
Ricardo Pella Cáceres Centro de Fertilidad y Ginecología del Sur. Biólogo con mención en genética
Alberto Vargas Lechuga Centro de Fertilidad y Ginecología del Sur. Médico Ginecólogo-Obstetra.
Livia Bartolo Durán Centro de Fertilidad y Ginecología del Sur. Bióloga

DOI:

https://doi.org/10.31403/rpgo.v58i86

Abstract

Oocytes in vitro maturation (IVM) usually used in patients with polycystic ovaries and polycystic ovary syndrome has been applied for the treatment of infertility in a woman with secondary amenorrea and history of anorexia nervosa. After repeated unsuccessful attempts of ovarian stimulation for conventional techniques of assisted reproduction, IVM treatment was begun by stimulating the ovaries with rFSH during the second, third and fourth day of the cycle and aspiring antral follicles on the eighth day. There were four cumulus-oocyte complexes that were cultivated in the maturation media for 36 hours in presence of FSH and hCG. Mature metaphase II oocytes were fertilized by intracytoplamatic sperm injection (ICSI) and embryos were frozen by vitrification on the second day. A month later, the endometrium was prepared with estradiol valerate. Thawed embryos were cultivated for 24 additional hours and three embryos were transferred. The patient achieved an evolutionary single gestation with active embryo.

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References

Cha KY, Koo JJ, Choi DH, Han SY, Yoon TK. Pregnancy after in vitro fertilization of human follicular oocytes collected from non stimulated cycles, their culture in vitro and their transfer in a donor oocyte program. Fertil Steril. 1991;55(1):109-13.

MacDougall MJ, Tan SL, Balen A, Jacobs HS. A controlled study comparing patients with and without polycystic ovaries undergoing in-vitro fertilization. Hum Reprod. 1993;8:233-7.

Brisden PR, WadaI, Tan SL, Balen A, Jacobs HS. Diagnosis, prevention and management of ovarian hyperstimulation syndrome. Br J Obstet Gynecol. 1995;102(10):767-72.

Kuwayama M, Vajta G, Ieda S, Kato O. Comparison of open and closed methods for vitrification of human embryos and the elimination of potential contamination. Reprod Biomed Online. 2005;11:608-14.

Reinblatt SL, Son WY, Shalom-Paz E, Holzer H. Controversies in IVM. J Assist Reprod Genet. 2011;6:525-30.

Rotterdam, ESHRE/ASRM -Sponsored PCOS Consensus Workshop Group. Revised 2003 concensus on diagnostic criteria and long term health risks related to polycystic ovary syndrome. Fertil Steril. 2004;81(1):19-25.

Son WY, Chung JT, Herrero B, Dean N , Demirtas E, HolzerH, Elizur S, Chian RC, Tan SL. Selection of the optimal day for oocyte retrieval based on the diameter of the dominant follicle in hCG-primed in vitro maturation cycles. Hum Reprod. 2008;23(12):2680-5.

Tan SL, Child TJ. In vitro maturation of oocytes from unstimulatedpolycyctic ovaries. Reprod Biomed Online. 2002;4 Suppl 1:18-23.

Son WY, Chung JT, Dermitas E, Holzer H , Sylvestre C, Buckett W, Chian RC, Tan SL. Comparison of in-vitro maturation cycles with and without in-vivo matured oocytes retrieved. Reprod Biomed Online. 2008;17(1):59-67.

Chian RC. In-vivo maturation of immature oocytes for infertile women with PCOS. Reprod Biomed Online. 2004;8:547-52.

Albertini DF, Combelles CM, Benecchi E, Carabatsos MJ. Cellular basis for paracrine regulation of ovarían follicle development. Reproduction. 2001;121:647-53.

De Vos M, Ortega-Hrepich C, Albuz FK, Guzman L , Polyzos NP, Smitz J, Devroey P. Clinical outcome of nonhCG- primed oocyte in vitro maturation treatment in patients with polycystic ovaries and polycyctic ovary syndrome. Fertil Steril. 2011;96(4):860-4.